October 31st, 2018

Agenda:

• Website 1: PTC Labs: http://learn.genetics.utah.edu/content/basics/ptc/
• Website 2: Great Overview of the lab:  http://bioinformatics.dnalc.org/ptc/animation/ptc.swf

Part I: How PCR Works: Please create a flowchart on how PCR functions. Use highlighters, markers, and/or modeling supply. 

• The Flow of the PCR: http://learn.genetics.utah.edu/content/labs/pcr/
• How Restriction Enzymes like HAEIII work: http://www.biology-pages.info/R/RestrictionEnzymes.html  
• https://learn.genetics.utah.edu/content/labs/gel/

PART II: SEQUENCING

Forward Primer 1:

CCTTCGTTTTCTTGGTGAATTTTTGGGATGTAGTGAAGAGGCGG

Reverse Primer 2:

AGGTTGGCTTGGTTTGCAATCATC

BLAST Website:

https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch